Showing posts with label Custom Antibody Custom Peptide Synthesis Custom SiRNA Synthesis. Show all posts
Showing posts with label Custom Antibody Custom Peptide Synthesis Custom SiRNA Synthesis. Show all posts

Thursday, April 7, 2011

Assay Analysis


Gene expression profiling or microarray analysis has enabled the measurement of thousands of genes in a single RNA sample. There are a variety of microarray platforms that have been developed to accomplish this and the basic idea for each is simple: a glass slide or membrane is spotted or "arrayed" with DNA fragments or oligonucleotides that represent specific gene coding regions.

Material Testing Services

Material testing services test, analyze, and certify a wide range of materials for purity, chemical compatibility, and environmental impact.

PNA Peptide Conjugates

As peptides can be inserted at the C-terminus or N-terminus. The positioning can be as follows: PNA-Peptide, Peptide1-PNA-Peptide2, and Peptide–PNA.

DNA Testing

The DNA of an individual is almost exactly the same in each and every somatic (non reproductive) cell. Sexual reproduction brings the DNA of both parents together randomly to create a unique combination of genetic material in a new cell, so the genetic material of an individual is derived from the genetic material of both their parents in roughly equal amounts.

Wednesday, February 9, 2011

TRIM25 Antibody

TRIM25 Antibody

Catalog# :4317

TRIM25, also known as estrogen responsive finger protein (EFP) is a member of the RING finger-B box-coiled coil family and is a downstream target of estrogen receptor a. Mice lacking this gene displayed an underdeveloped uterus and reduced estrogen responsiveness demonstrating that this protein is essential for estrogen dependent proliferation. TRIM25 expression is increased in breast carcinoma where it promotes the growth of breast tumor by functioning as an E3 ubiquitin ligase that targets the negative cell cycle checkpoint 14-3-3s. Recent reports have indicated that TRIM25 E3 ubiquitin ligase is essential for the retinoic-acid-inducible gene 1 (RIG-1) signaling pathway to elicit host antiviral innate immunity.

Additional Names : TRIM25 (NT), Tripartite motif-containing 25, estrogen responsive finger protein, EFP
Description
Left: Western blot analysis of TRIM25 in human thymus tissue lysate in (A) the absence and (B) absence of blocking peptide with TRIM25 antibody at 1 µg/ml.

Below Immunocytochemistry of TRIM25 in HeLa cells with TRIM25 antibody at 5 µg/ml.

Other Product Images

Source :TRIM25 antibody was raised against a 15 amino acid peptide from near the amino terminus of human TRIM25.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : TRIM25 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : TRIM25 antibody can be used for detection of TRIM25 by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB, ICC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4317P - TRIM25 Peptide
Long-Term Storage : TRIM25 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1314 - Human Thymus Tissue Lysate
Species Reactivity : H
GI Number : 68160937
Accession Number : NP_005073
Short Description : (NT) estrogen responsive finger protein
References
1. Inoue S, Orimo A, Hosoi T, et al. Genomic binding-site cloning reveals an estrogen-responsive gene that encodes a RING finger protein. Proc. Natl. Acad. Sci. USA 1993; 11117-20.
2. Orimo A, Inoue S, Minowa O, et al. Underdeveloped uterus and reduced estrogen responsiveness in mice with disruption of the estrogen-responsive finger protein gene, which is a direct target of estrogen receptor a. Proc. Natl. Acad. Sci. USA 1999; 96:12027-32.
3. Thomson SD, Ali S, Pickles L, et al. Analysis of estrogen-responsive finger protein expression in benign and malignant human breast. Int. J. Cancer 2001; 91:152-8.
4. Urano T, Saito T, Tsukui T, et al. Efp targets 14-3-3s for proteolysis and promotes breast tumour growth. Nature 2002; 417:871-5.

Monday, January 31, 2011

Luminex Assays

Bio-Synthesis offers sample analysis services utilizing its kits for the Luminex System. All testing is performed in our state of the art facility in North Texas. There is a 35 sample minimum required per panel request. Multiplex Antibody Bio-Synthesis will perform the necessary assays and deliver a written report for each batch of samples. Any special instructions regarding handling samples or desired analysis should be communicated to us at the time the samples are provided. Unless otherwise requested by the investigators, each sample will be analyzed in duplicate.

Luminex Assays
Luminex assays are based on xMAP technology (multi-analyte profiling beads) enabling the detection and quantitation of multiple RNA or protein targets simultaneously. The xMAP system combines a flow cytometer, fluorescent-dyed microspheres (beads), lasers and digital signal processing to effectly allow multiplexing of up to 100 unique assays within a single sample.

Multiplex Assays
Offers multiplex assays for quantitation and detection of soluble proteins and intracellular pathway markers, using Luminex bead-based technology. These assays allow simultaneous measurement of multiple targets in each sample, with superior performance and reproducible results lot after lot.

Monday, November 8, 2010

JMJD5 Antibody

JMJD5 Antibody

Catalog# : 5381

The jumonji domain-containing protein (JMJD) family is defined by the presence of the JmjC domain that is observed in several diverse species. While several JMJD proteins have been identified as being involved in chromatin regulation, histone demethylation and development, the function of JMJD5 has not been identified. JMJD5 is expressed in multiple tissues.
Additional Names : JMJD5, Jumonji domain-containing protein 5


Description
Left: Western blot analysis of JMJD5 in human liver tissue lysate with JMJD5 antibody at (A) 1 and (B) 2 µg/ml.





Source : JMJD5 antibody was raised against an 18 amino acid peptide from near the carboxy terminus of human JMJD5.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : Polyclonal
Host : JMJD5 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : JMJD5 antibody can be used for detection of JMJD5 by Western blot at 1 - 2 µg/ml.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat.No. 5381P - JMJD5 Peptide
Long-Term Storage : JMJD5 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1304 - Human Liver Tissue Lysate
Species Reactivity :H, M, R
GI Number : 223942018
Accession Number : NP_001138820
Short Description : Jumonji domain-containing protein 5
References
1. Takeuchi T, Watanabe Y, Takano-Shimizu T, et al. Roles of jumonji and jumonji family genes in chromatin regulation and development. Dev. Dyn. 2006; 235:2449-59.

Friday, October 1, 2010

CTRP7 Antibody

CTRP7 Antibody

Catalog# : 3673

Adipose tissue of an organism plays a major role in regulating physiologic and pathologic processes such as metabolism and immunity by producing and secreting a variety of bioactive molecules termed adipokines (reviewed in 1). One highly conserved family of adipokines is adiponectin/ACRP30 and its structural and functional paralogs, the C1q/tumor necrosis factor-?-related proteins (CTRPs) 1-7 (2). Unlike adiponectin, which is expressed exclusively by differentiated adipocytes, the CTRPs are expressed in a wide variety of tissues (3). These proteins are thought to act mainly on liver and muscle tissue to control glucose and lipid metabolism. An analysis of the crystal structure of adiponectin revealed a structural and evolutionary link between TNF and C1q-containing proteins, suggesting that these proteins arose from a common ancestral innate immunity gene (4). Like the other members of the adiponectin and CTRP protein family, the mature CTRP7 is secreted and can be found in the organism’s circulatory system (1).
Additional Names : CTRP7
Description
Left: Western blot of recombinant CTRP7: (A) 5 ng, (B) 25 ng, and (C) 50 ng with CTRP2 antibody at 1 ug/ml.

Below: Immunocytochemistry of CTRP7 in 293 cells with CTRP7 antibody at 10 µg/ml.

Other Product Images

Source : Rabbit anti-CTRP7 polyclonal antibody was raised against recombinant human CTRP7.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : CTRP7 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Recombinant mouse CTRP7 (Genbank accession No. AAY21932).
Application : CTRP7 antibody can be used for the detection of CTRP7 by Western blot at 0.5 µg/ml.These proteins are often highly modified post-translationally and migrate in SDS-PAGE at positions other than their predicted size.
Tested Application(s) : E, WB, ICC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat.No. 3673P - CTRP7 Peptide
Long-Term Storage : CTRP7 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1210 - 293 Cell Lysate
Species Reactivity :H, M, R
GI Number : 62913965
Accession Number : AAY21932
Short Description : a novel adipokine
References
1. Fantuzzi G. Adipose tissue, adipokines, and inflammation. J. Allergy Clin. Immunol. 2005; 115:911-9.
2. Tsao T-S, Lodish HF, and Fruebis J. ACRP30, a new hormone controlling fat and glucose metabolism. Euro. J. Pharmacol. 2002; 440:213-21.
3. Wong GW, Wang J, Hug C, et al. A family of Acrp30/ adiponectin structural and functional paralogs. Proc. Natl. Acad. Sci. USA 2004; 101:10302-7.
4. Shapiro L and Scherer PE. The crystal structure of a complement-1q family protein suggests an evolutionary link to tumor necrosis factor. Curr. Biol. 1998; 8:335-8.

Wednesday, September 29, 2010

APRIL Antibody

APRIL Antibody

Catalog# : 2223

Members in the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated APRIL (for a proliferation-inducing ligand), TALL-2 (for TNF- and ApoL-related Leukocyte-expressed Ligand 2), and TRDL-1alpha (for TNF related death ligand 1a) in human and mouse (1-4). Two receptors for APRIL were recently identified and designated TACI and BCMA (5-7). APRIL stimulates B and T cell proliferation, triggers humoral immune responses, activates NF-kappaB, and induces cell death (1-7). APRIL and its close relative of BlyS and their receptors BCMA and TACI are involved in diseases of autoimmunity and cancer (8,9). Additional Names : APRIL (ED), APRIL Description Left: Western blot analysis of APRIL expression in K562 cells with APRIL antibody at (A) 5 and (B) 10 µg/ml. Source : APRIL antibody was raised against a synthetic peptide corresponding to amino acids near the center of human APRIL. Purification : Affinity chromatography purified via peptide column Clonality and Clone : This is a polyclonal antibody. Host : APRIL antibody was raised in rabbit. Please use anti-rabbit secondary antibodies. Immunogen : Human APRIL (Extracellular Domain) Peptide (Cat. No. 2223P) Application : APRIL can be used for detection of APRIL by Western blot at 4-5 µg/ml.A 27 kDa band can be detected. Tested Application(s) : E, WB Buffer : Antibody is supplied in PBS containing 0.02% sodium azide. Blocking Peptide : Cat. No. 2223P - APRIL Peptide Long-Term Storage : APRIL antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Positive Control 1. Cat. No. 1204 - K562 Cell Lysate Species Reactivity :H GI Number : 4507599 Accession Number : NP_003799 Short Description : (ED) A new member in the TNF family References 1. Hahne M, Kataoka T, Schroter M, et al. APRIL, a new ligand of the tumor necrosis factor family, stimulates tumor cell growth. J Exp Med. 1998;188(6):1185-90. 2. Shu HB, Hu WH, Johnson H. TALL-1 is a novel member of the TNF family that is down-regulated by mitogens. J Leukoc Biol. 1999;65(5):680-3. 3. Kelly K, Manos E, Jensen G, et al. APRIL/TRDL-1, a tumor necrosis factor-like ligand, stimulates cell death. Cancer Res. 2000;60(4):1021-7. 4. Yu G, Boone T, Delaney J, et al. APRIL and TALL-I and receptors BCMA and TACI: system for regulating humoral immunity. Nat Immunol. 2000;1(3):252-6.

Wednesday, August 18, 2010

OMI Antibody

OMI Antibody

Catalog# : 3051

Inhibitor of apoptosis proteins (IAPs) were initially identified in baculoviruses as proteins that inhibit apoptosis of the host cells to allow time for viral replication (1). Cellular homologues containing at least one baculoviral IAP repeat (BIR) motif essential for their anti-apoptosis activity have been identified in yeasts and higher organisms and often act by binding and inhibiting processed caspases (reviewed in 2). The activity of these proteins can be modulated by the expression of proteins such as Smac/DIABLO and XAF-1 which displace or prevent the binding of caspases by IAPs (reviewed in 3). Recently, a mitochondrial serine protease termed Omi/HtrA2 has been found to bind IAPs (4). Similar to Smac, Omi possesses a conserved IAP-binding motif, but acts to cleave IAPs to irreversibly inactivate IAPs and promote apoptosis (5).

Additional Names : OMI (C2), HtrA2
Description
Left: Western blot analysis of OMI in U937 lysate with Omi antibody at (A) 0.5, (B) 1, and (C) 2 µg/ml.

Below: Immunohistochemistry of OMI in human liver tissue with OMI antibody at 2 µg/ml.

Other Product Images
Source : OMI antibody was raised against a peptide corresponding to 16 amino acids near the C-terminus of human Omi.

Purification : Ion exchange chromatography purified

Clonality and Clone : This is a polyclonal antibody.

Host : OMI antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Immunogen : Human OMI / HtrA2 (C-Terminus 2) Peptide (Cat. No. 3051P)

Application : OMI antibody can be used for detection of OMI by Western blot at 0.5 to 1 µg/ml.

Tested Application(s) : E, WB, IHC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 3051P - OMI Peptide

Long-Term Storage : OMI antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1. Cat. No. 1215 - U937 Cell Lysate

Species Reactivity :H

GI Number : 5870865

Accession Number : AAB94569

Short Description : (C2) IAP inhibitor

References

1. Crook NE, Clem RJ, and Miller LK. An apoptosis inhibiting baculovirus gene with a zinc finger like motif. J. Virol. 1993; 67:2168-2174.
2. Liston P, Fong WG, and Korneluk RG. The inhibitors of apoptosis: there is more to life than Bcl2. Oncogene 2003; 22:8568-80.
3. Vaux DL and Silke J. Mammalian mitochondrial IAP binding proteins. Biochem. Biophys. Res. Comm. 2003; 304:499-504.
4. Suzuki Y, Imai Y, Nakayama H, et al. A serine protease, HtrA2, is released from the mitochondria and interacts with XIAP, inducing cell death. Mol. Cell 2001; 8:613-21.