Friday, June 10, 2011
Ribosomal RNA Primers
Bio-Synthesis, founded in 1984, is an international provider of genomic services established around the core business lines oligonucleotide such as RNR primers and probes and gene synthesis. Base, ribose and backbone modifications and reporter groups like dyes and haptens are routinely incorporated in DNA, RNA, Constrained Nucleotide, and PNA. Through innovative production process and synthesis know-how, our services are the best in their class, providing you with innovative and trustworthy solutions to accelerate your research.
A primer is a strand of nucleic acid that serves as a starting point for DNA replication. They are required because the enzymes that catalyze replication, DNA polymerases, can only add new nucleotides to an existing strand of DNA. The polymerase starts replication at the 3'-end of the primer, and copies the opposite strand.
PCR Primer Design
The melting temperature of a primer is defined as the temperature at which 50% of that same DNA molecule species form a stable double helix and the other 50% have been separated to single strand molecules. The melting temperature required increases with the length of the primer. Primers that are too short would anneal at several positions on a long DNA template, which would result in non-specific copies. On the other hand, the length of a primer is limited by the temperature required to melt it. Melting temperatures that are too high, i.e., above 80°C, can also cause problems since the DNA polymerases used for PCR are less active at such temperatures.
Ribosomes (from ribonucleic acid and "Greek: soma (meaning body)") are complexes of RNA and protein that are found in all cells. Ribosomes from bacteria and archaea are smaller than the ribosomes from eukaryotes, although all three domains of life have significantly different ribosomes. Interestingly, the ribosomes in the mitochondrion of eukaryotic cells resemble those in bacteria, reflecting the evolutionary origin of this organelle.
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