Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappaB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kappaB associates with IkappaB proteins in the cell cytoplasm, which inhibit NF-kappaB activity. IkappaB is phosphorylated by IkappaB kinase (IKK) complex that contains IKKalpha, IKKbeta, and IKKgamma. A novel molecule that associates with and activates IKK was recently identified and designated CIKS (for connection to IKK and SAPK/JNK) and Act1 (for NF-kappaB activator 1) (1,2). CIKS directly interacts with IKKgamma. CIKS/Act1 also activates activating transcription factor (ATF) and activator protein 1 (AP-1) through Jun kinase (JNK). These results indicate that CIKS/Act1 is involved in the inflammation and stress responses. CIKS/Act1 is ubiquitously expressed in human tissues.
Additional Names: CIKS (NT), CIKS/Act1
Left: Western blot analysis of CIKS expression in human lung (lane A) and placenta (lane B) tissue lysates with CIKS antibody at 1 µg /ml.
Below: Immunohistochemistry of CIKS in human lung tissue with CIKS antibody at 5 µg/ml.
Other Product Images
Source:CIKS antibody was raised against a synthetic peptide corresponding to amino acids 2 to 15 of human CIKS .
Purification: Affinity chromatography purified via peptide column
Clonality and Clone: This is a polyclonal antibody.
Host: CIKS antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen: Human CIKS (N-Terminus) Peptide (Cat. No. 2501P)
Application: CIKS antibody can be used for detection of CIKS by Western blot at 0.5 to 1 µg/ml.A band at approximately 63 kDa can be detected.
Tested Application(s): E, WB, IHC
Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide:Cat. No. 2501P - CIKS Peptide
Long-Term Storage: CIKS antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1. Cat. No. 1309 - Human Placenta Lysate
2. Cat. No. 1302 - Human Lung Tissue Lysate
Species Reactivity: H
GI Number: 10242289
Accession Number: AF274303
Short Description: (NT) A novel NF-kappaB activator
1. Leonardi A, Chariot A, Claudio E, Cunningham K, Siebenlist U. CIKS, a connection to Ikappa B kinase and stress-activated protein kinase. Proc Natl Acad Sci USA . 2000;97(19):10494-9.
2. Li X, Commane M, Nie H, Hua X, Chatterjee-Kishore M, Wald D, Haag M, Stark GR. Act1, an NF-kappa B activating protein. Proc Natl Acad Sci USA . 2000;97(19):10489-93.