Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. Grik1, also known as glutamate receptor 5, belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. Grik1 is expressed in GABAergic interneurons of the hippocampus and are thought to participate in the formation of various subtypes of kainate receptors with Grik2 and KA2. Stimulation of Grik1 leads to intracellular calcium release and activation of protein kinase C. Excessive activation has been associated with psychiatric, neurological and neurodegenerative diseases. Numerous isoforms of Grik1 are known to exist and may be subject to RNA editing within the second transmembrane domain, which is thought to alter the properties of ion flow.
Additional Names : Grik1 (CT), Glutamate receptor ionotropic kainate 1, glutamate receptor 5, gluR5, excitatory amino acid receptor 3, eea3
Left: Western blot analysis of Grik1 in P815 cell lysate with Grik1 antibody at (A) 0.5, (B) 1 and (C) 2 µg/ml.
Below: Immunohistochemical staining of human brain tissue using Grik1 antibody at 2.5 µg/ml.
Other Product Images
Source :Grik1 antibody was raised against a 15 amino acid peptide near the carboxy terminus of the human Grik1.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : Grik1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : Grik1 antibody can be used for detection of Grik1 by Western blot at 0.5 – 1 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4381P - Grik1 Peptide
Long-Term Storage : Grik1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1286 - P815 Cell Lysate
Species Reactivity : H, M
GI Number : 729597
Accession Number : P39086
Short Description : (CT) Glutamate receptor 5
1. Tanaka K. Functions of glutamate transports in the brain. Neurosci. Res. 2000; 37:15-9.
2. Pinheiro P and Mulle C. Kainate receptors. Cell Tissue Res. 2006; 326:457-82.
3. Bureau I, Dieudonne S, Coussen F, et al. Kainate receptor-mediated responses in the CA1 field of wild-type and GluR6-deficient mice. J. Neurosci. 1999; 19:653-63.
4. Christensen JK, Paternain AV, Selak S, et al. A mosaic of functional kainate receptors in hippocampal interneurons. J. Neurosci. 2004; 24:8986-93.