CAPS2 and the related protein CAPS1 encode novel neural/endocrine-specific cytosolic and peripheral membrane proteins. Both are essential components of the synaptic vesicle priming machinery and are required for the Ca2+-regulated exocytosis of secretory vesicles; CAPS-deficient neurons contain no or very few fusion competent synaptic vesicles, causing a selective impairment of fast phasic transmitter release. Like CAPS1, numerous isoforms of CAPS2 are known to exist. These isoforms show distinct expression patterns in the brain. For example, CAPS2b showed high expression in the developing cerebellum. Furthermore, one version of CAPS2 mRNA that lacks exon 3 is detected in some autistic patients, suggesting that the differential expression pattern of CAPS2 is involved in neuronal development. This CAPS2 antibody is predicted to be specific to CAPS2 and not recognize CAPS1.
Additional Names : CAPS2 (IN), Calcium-dependent secretion activator 2, CADPS2
Left: Western blot analysis of CAPS2 in human brain tissue lysate with CAPS2 antibody at (A) 0.5 and (B) 1 µg/ml.
Below:Immunohistochemistry of CAPS2 in human brain with CAPS2 antibody at 5 µg/ml.
Other Product Images
Source :CAPS2 antibody was raised against a 19 amino acid peptide near the center of the human CAPS2.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : CAPS2 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : CAPS2 antibody can be used for detection of CAPS2 by Western blot at 0. 5 – 1 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4565P - CAPS2 Peptide
Long-Term Storage : CAPS2 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1303 - Human Brain Tissue Lysate
Species Reactivity : H, M, R
GI Number : 148839294
Accession Number : NP_060424
Short Description : (IN) Calcium-dependent secretion activator 2
1. Walent JH, Porter BW, and Martin TF. A novel 145 kD brain cytosolic protein reconstitutes Ca(2+)-regulated secretion in permeable neuroendocrine cells. Cell 1992; 70:765-775.
2. Juckusch WJ, Speidel D, Sigler A, et al. CAPS-1 and CAPS-2 are essential synaptic vesicle priming proteins. Cell 2007; 131:796-808.
3. Sadakata T, Washida M, and Furuichi T. Alternative splicing variants in mouse CAPS2: differential expression and functional properties of splicing variants. BMC Neurosci. 2007; 8:25-35.