Monday, April 11, 2011
BICD2 is the second human homolog discovered to the Drosophila Bicaudal-D protein that forms part of the cytoskeleton and mediates the correct sorting of mRNAs for oocyte- and axis-determining factors during oogenesis. Similar to the highly homologous protein BICD1, BICD2 can bind to dynein-dynactin complex, primarily through the dynamitin subunit of dynactin. The C-terminus of BICD2 targets the protein to the Golgi complex while the N-terminal domain of BICD2 co-immunoprecipitates with cytoplasmic dynein, suggesting BICD2 plays a role in the dynein-dynactin interaction on the surface of membranous organelles. Mice engineered to overexpress the BICD2 amino terminal domain in neurons developed amyotrophic lateral sclerosis (ALS)-like features such as Golgi fragmentation, neurofilament swelling in proximal axons, etc., suggesting that impaired dynein/dynactin function may explain some of the pathological features observed in ALS patients. This BICD2 antibody will not cross-react with BICD1.
Left: Western blot analysis of BICD2 in A549 cell lysate with BICD2 antibody at (A) 1 and (B) 2 µg/ml।
Source :BICD2 antibody was raised against a 13 amino acid peptide from near the carboxy terminus of human BICD2.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : BICD2 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : BICD2 antibody can be used for detection of BICD2 by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4675P - BICD2 Peptide
Long-Term Storage : BICD2 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1.Cat. No. 1203 - A549 Cell Lysate
Species Reactivity : H, M, R
GI Number : 57208854
Accession Number : CAI41013
Short Description : (CT) Bicaudal D homolog 2
1. Holland PM, Milne A, Garka K, et al. Purification, cloning, and characterization of Nek8, a novel NIMA-related kinase, and its candidate substrate Bicd2. J. Biol. Chem. 2002; 277:16229-40.
2. Hoogenraad CC, Akhmanova A, Howell SA, et al. Mammalian golgi-associated Bicaudal-D2 functions in the dynein-dynactin pathway by interacting with these complexes. EMBO J. 2001; 4041-54.
3. Teuling E, van Dis V, Wulf PS, et al. A novel mouse model with impaired dynein/dynactin function develops amyotrophic lateral sclerosis (ALS)-like features in motor neurons and improves lifespan in SOD1-ALS mice. Hum. Mol. Genet. 2008; 17:2849-62.