Sunday, April 10, 2011
BAP3 was initially identified through interaction in a yeast two-hybrid system with the brain-specific angiogenesis inhibitor 1, a p53-target gene that encodes a seven-span transmembrane protein member of the secretin receptor family. BAP3 is predominantly expressed in the brain and possess high homology with Munc13 and synaptotagmin, suggesting that BAP3 may play a role in regulating neurotransmitter release. Recent experiments have shown that BAP3 is induced in certain tumors such as desmoplastic small round cell tumor. Ectopic expression of BAP3 in tumor cells dramatically enhances growth in low serum conditions and colony formation in soft agar, suggesting that the regulated exocytotic pathway may play a role in cancer cell proliferation. BAP3 is known to have two isoforms; this BAP3 antibody will recognize only isoform 2. Lower molecular weight bands may represent cleavage or degradation products.
Left: Western blot analysis of BAP3 in SK-N-SH cell lysate with BAP3 antibody at (A) 1 and (B) 2 µg/ml.
Below: Immunohistochemical staining of human brain tissue using BAP3 antibody at 5 µg/ml.
Other Product Images
Source :BAP3 antibody was raised against a 13 amino acid peptide from near the amino terminus of human BAP3.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : BAP3 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : BAP3 antibody can be used for the detection of BAP3 by Western blot at 2 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4505P - BAP3 Peptide
Long-Term Storage : BAP3 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1220 - SK-N-SH Cell Lysate
Species Reactivity : H
GI Number : 119606077
Accession Number : EAW85671
Short Description : (NT2) BAI1-associated protein 3
1. Shiratsuchi T, Oda K, Nishimori H, et al. Cloning and characterization of BAP (BAI-associated protein 3), a C2 domain-containing protein that interacts with BAI1. Biochem. Biophys. Res. Comm. 1998; 251:158-65.
2. Palmer RE, Lee SB, Wong JC, et al. Induction of BAIAP3 by the EWS-WT1 chimeric fusion implicates regulated exocytosis in tumorigenesis. Cancer Cell 2002; 2:497-505.