Accumulation of the amyloid-beta peptide (Abeta) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer’s disease. The beta−amyloid protein precursor (APP) is cleaved by one of two beta−secretases (BACE and BACE2), producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for gamma−secretase to generate the 4 kDa amyloid-beta peptide (Abeta), which is deposited in the Alzheimer’s disease patients’ brains. Recently, Death Receptor 6 (DR6) was found to interact with an amino-terminal fragment of the Beta-amyloid protein (N-APP) in neurons, activating a caspase 6-dependent apoptotic event leading to axonal degeneration and pruning during development, suggesting that these two proteins are involved in neural development and may possibly play a role in Alzheimer’s disease.
Additional Names : APP (NT2), Amyloid Precursor Protein, amyloid-beta precursor protein
Left: Western blot analysis of APP in rat brain tissue lysate with APP antibody at (A) 1 and (B) 2 µg/ml.
Below:Immunohistochemistry of APP in mouse brain tissue with APP antibody at 2.5 μg/ml.
Other Product Images
Source :APP antibody was raised against an 18 amino acid peptide near the amino terminus of human APP.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : APP antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : APP antibody can be used for detection of APP by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 5143P - APP Peptide
Long-Term Storage : APP antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1463 - Rat Brain Tissue Lysate
Species Reactivity : H, M, R
GI Number : 112927
Accession Number : P05067
Short Description : (NT2) Amyloid precursor protein
1. Ponte P, Gonzalez-DeWhitt P, Schilling J, et al. A new A4 amyloid mRNA contains a domain homologous to serine proteinase inhibitors. Nature 1988; 331:525-77.
2. Selkoe DJ. Cell biology of the amyloid beta-protein precursor and the mechanism of Alzheimer's disease. Annu Rev Cell Biol 1994; 10:373-403.
3. Nikolaev A, McLaughlin T, O’Leary DD, et al. APP binds DR6 to trigger axon pruning and neuron death via distinct caspases. Nature 2009; 457:981-9.