Tuesday, March 29, 2011
Phospho Specific Antibodies
Bio-Synthesis offers the production of phosphorylation state-specific polyclonal antibodies. With over 20 years of experience in phosphorylated peptide synthesis and phosphor specific antibodies that recognize only the activated form of the protein target. We routinely assist clients with antigen peptide predicition using our sophisticated software to determine the best regions on the protein of interest based on antigenicity hydrophobicity and hydrophilicity, exibility, secondary structure and aggregation potential. The complexity of manufacturing the peptide is also considered. Once the sequence has been determined, peptide synthesis, conjugation, polyclonal or monclonal antibody production, affinity purifcation, fragmentation, and immunoassay development can all perform in our state-of-art facilities.
Protein phosphorylation-dephosphorylation is one of the major signaling mechanisms for modulating the functional properties of proteins involved in gene expression, cell adhesion, cell cycle, cell proliferation, and differentiation.
The most common peptide modification used for polyclonal antibody production is the phosphorylation of amino acids; which is accomplished immunizing host animals with a short synthetic peptide containing the phosphoryl (PO(3)) group on the residue of interest.