MPYS is a recently identified plasma membrane tetraspanner that is associated with major histocompatibility complex class II (MHC-II) and mediates its transduction of apoptotic signals. It has also been found to be associated with VISA, a mitochondrial protein that acts as an adaptor in virus-triggered signaling. MPYS also interacts with IRF3 and recruits the kinase TBK1 to the VISA-associated complex, acting as a critical mediator of virus-triggered IRF3 activation and interferon (IFN) expression. It is thought that the binding of nucleic acid to the innate immune protein RIG-I causes complex formation between RIG-I, VISA, and MPYS. This complex then recruits TBK1 to phosphorylate IRF3 which then directly activates IFN transcription. At least three isoforms of MPYS are known to exist.
Additional Names : MPYS, Transmembrane protein 173, TMEM173, ERIS, MITA, STING
Left: Western blot analysis of MPYS in A-20 cell lysate with MPYS antibody at (A) 1 and (B) 2 µg/ml.
Source :MPYS antibody was raised against a 17 amino acid peptide near the carboxy terminus of human MPYS.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : Polyclonal
Host : Rabbit
Application : MPYS antibody can be used for detection of MPYS by Western blot at 1 - 2 µg/ml.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 5269P - MPYS Peptide
Long-Term Storage : MPYS antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1288 - A20 Cell Lysate
Species Reactivity : H, M
GI Number : 38093659
Accession Number : NP_938023
Short Description : Transmembrane protein 173
1. Jin L, Waterman PM, Jonscher KR, et al. MPYS, a novel membrane tetraspanner, is associated with major histocompatibility complex class II and mediates transduction of apoptotic signals. Mol. Cell. Biol. 2008; 28:5014-26.
2. Zhong B, Yang Y, Li S, et al. The adaptor protein MITA links virus-sensing receptors to IRF3 transcription factor activation. Immunity 2008; 29:538-50.