Axin is a key modulator of the Wnt/beta-catenin pathway and acts as a scaffold protein in phosphorylating and degrading cytoplasmic beta-catenin. ZBED3, a member of the Zinc finger BED-type domain containing superfamily, has been recently identified as an Axin-interacting protein and has been suggested to modulate the Wnt/beta-catenin signaling pathway. RNA interference (RNAi) experiments targeting ZBED3 RNA attenuated Wnt-induced beta-catenin accumulation, lymphoid enhancer binding factor-1-dependent luciferase reporter activity, and Wnt gene expression. Despite its predicted molecular weight, ZBED3 often migrates at a higher position in SDS-PAGE. This antibody is predicted to have no cross-reactivity to ZBED1 or ZBED2.
Additional Names : ZBED3 (CT), Zinc finger BED-type domain containing 3
Left:Western blot analysis of ZBED3 in human lung tissue lysate with ZBED3 antibody at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide.
Other Product Images
Source :ZBED3 antibody was raised against a 15 amino acid peptide from near the carboxy terminus of human ZBED3.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : ZBED3 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : ZBED3 antibody can be used for detection of ZBED3 by Western blot at 1 - 2 µg/ml.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 5113P - ZBED3 Peptide
Long-Term Storage : ZBED3 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1302 - Human Lung Tissue Lysate
Species Reactivity : H
GI Number : 14150185
Accession Number : NP_115743
Short Description : (CT) Zinc finger BED-type domain containing 3
1. Chen T, Li M, Ding Y, et al. Identification of Zinc-finger BED domain containing 3 (Zbed3) as a novel axin-interacting protein that activates Wnt/b-catenin signaling. J. Biol. Chem. 2009; 284:6683-9.