TRIM25, also known as estrogen responsive finger protein (EFP) is a member of the RING finger-B box-coiled coil family and is a downstream target of estrogen receptor a. Mice lacking this gene displayed an underdeveloped uterus and reduced estrogen responsiveness demonstrating that this protein is essential for estrogen dependent proliferation. TRIM25 expression is increased in breast carcinoma where it promotes the growth of breast tumor by functioning as an E3 ubiquitin ligase that targets the negative cell cycle checkpoint 14-3-3s. Recent reports have indicated that TRIM25 E3 ubiquitin ligase is essential for the retinoic-acid-inducible gene 1 (RIG-1) signaling pathway to elicit host antiviral innate immunity.
Additional Names : TRIM25 (NT), Tripartite motif-containing 25, estrogen responsive finger protein, EFP
Left: Western blot analysis of TRIM25 in human thymus tissue lysate in (A) the absence and (B) absence of blocking peptide with TRIM25 antibody at 1 µg/ml.
Below Immunocytochemistry of TRIM25 in HeLa cells with TRIM25 antibody at 5 µg/ml.
Other Product Images
Source :TRIM25 antibody was raised against a 15 amino acid peptide from near the amino terminus of human TRIM25.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : TRIM25 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : TRIM25 antibody can be used for detection of TRIM25 by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB, ICC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4317P - TRIM25 Peptide
Long-Term Storage : TRIM25 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1314 - Human Thymus Tissue Lysate
Species Reactivity : H
GI Number : 68160937
Accession Number : NP_005073
Short Description : (NT) estrogen responsive finger protein
1. Inoue S, Orimo A, Hosoi T, et al. Genomic binding-site cloning reveals an estrogen-responsive gene that encodes a RING finger protein. Proc. Natl. Acad. Sci. USA 1993; 11117-20.
2. Orimo A, Inoue S, Minowa O, et al. Underdeveloped uterus and reduced estrogen responsiveness in mice with disruption of the estrogen-responsive finger protein gene, which is a direct target of estrogen receptor a. Proc. Natl. Acad. Sci. USA 1999; 96:12027-32.
3. Thomson SD, Ali S, Pickles L, et al. Analysis of estrogen-responsive finger protein expression in benign and malignant human breast. Int. J. Cancer 2001; 91:152-8.
4. Urano T, Saito T, Tsukui T, et al. Efp targets 14-3-3s for proteolysis and promotes breast tumour growth. Nature 2002; 417:871-5.