Sunday, February 6, 2011
PNA Fish Applications
Bio-Synthesis, founded in 1984, is an international provider of genomic services established around the core business lines oligonucleotide and gene synthesis. Base, ribose and backbone modifications and reporter groups like dyes and haptens are routinely incorporated in DNA, RNA, Constrained Nucleotide, and PNA. Through innovative production process and synthesis know-how, our services are the best in their class, providing you with innovative and trustworthy solutions to accelerate your research.
Growing bacteria and yeast cells produce an abundance of ribosomal RNAs that contain regions of highly conserved, species-specific sequences and are therefore ideal targets for identification assays such as FISH. However, the target sequences are frequently located in highly structured regions of the rRNA which are virtually inaccessible to DNA probes.
Antisense peptide nucleic acids (PNAs) can specifically inhibit Escherichia coli gene expression and growth and hold promise as anti-infective agents and as tools for microbial functional genomics. Here we demonstrate that chemical modification improves the potency of standard PNAs.